ENY 6822C - MOLECULAR BIOLOGY TECHNIQUES OF INVERTEBRATES AND THEIR PATHOGENS
Summer A or Summer B of even years.
8:00 a.m. - 12:00 noon
Room 3130
Bldg. 970, Natural Area Drive
Updated August 2013

Instructor and Office Hours:
Dr. James E. Maruniak, marun@ufl.edu
Room 3109 Steinmetz Hall (Entomology Building)
Bldg. 970 Natural Area Drive
P.O. Box 110620
TEL 352-273-3960
FAX 352-392-0190

Office hours: Contact instructor via phone, email or after class to arrange a meeting.

Course Description: This course is designed to give practical experience for graduate students in learning, understanding and applying molecular biology techniques for their own research projects. The students will extract the DNA from their own invertebrate or pathogen of interest. That material will be used for further experiments involving restriction endonuclease (REN) digestions, DNA cloning, DNA Southern blotting, PCR amplification, DNA sequencing and DNA data analysis using GCG computer programs, phylogeny using PAUP and other programs. Students will also learn cell culture handling procedures. Lectures will provide the fundamental background necessary for understanding molecular biology pertinent to the methodology that will be used. Since new molecular methodologies are constantly introduced and modified, the course techniques will be updated as needed.

Prerequisites: Any basic course in genetics, biochemistry or molecular biology.

Textbook: None. Protocols and handouts will be given.

Assignments and Methods by which the Student will be Evaluated and Grades Determined: The course grade will be determined by how well the student maintains the laboratory notebook (30%), preparation of protocols (10%), experimental results (20%), participation and discussion (15%) and the presentation of an experimental project involving molecular techniques (25%).

Critical Dates for Exams or Other Work: There are no written exams. The student project presentations will be scheduled during the semster.

Policy Related to Class Attendance: Students are expected to attend all class sessions. Experimental procedures each day build upon the next for manipulation of DNA.

Class Demeanor Expected by Instructor: Please be considerate of your classmates by not chatting during lecture. The banging of doors is very distracting to both students and professor, therefore please arrive on time and do not leave early. Turn off cell phones and beepers before coming into classroom. UF rules prohibit your having food or drinks in classrooms. Use of tobacco products (in any form) in the classroom is prohibited.

Additional General Information: The following information applies to all courses at the University of Florida.

We, the members of the University of Florida community, pledge to hold ourselves and our peers to the highest standard of honesty and integrity.

Academic Honesty: As a result of completing the registration form at the University of Florida, every student has signed the following statement: "I understand that the University of Florida expects its students to be honest in all their academic work. I agree to adhere to this commitment to academic honesty and understand that my failure to comply with this commitment may result in disciplinary action up to and including expulsion from the University."

Copyrighted Materials and Software Use: All students are required and expected to obey the laws and legal agreements governing copyrighted material and software use. Failure to do so can lead to monetary damages and/or criminal penalties for the individual violator. Because such violations are also against University policies and rules, disciplinary action will be taken as appropriate.

Accommodations for Students with Disabilities: Students requesting classroom accommodation must first register with the Dean of Students Office. The Dean of Students will provide documentation to the student who must then provide this documentation to the Instructor when requesting accommodation.

University Counseling Services: Resources are available on-campus for students having personal problems or lacking clear career and academic goals which interfere with their academic performance. These resources include:

1. University Counseling Center, 301 Peabody Hall, 392-1575, personal and career counseling;
2. Student Mental Health, Student Health Care Center, 392-1171, personal counseling;
3. Sexual Assault Recovery Services (SARS), Student Health Care Center, 392-1161, sexual counseling; and
4. Career Resource Center, Reitz Union, 392-1601, career development assistance and counseling.